• The nasopharyngeal swab is collected
  • RNA is extracted
  • A single step RT-PCR is done
  • The FELUDA mix is prepared by incubating the dead FnCas9 protein, guide RNA and the amplified viral DNA
  • The dip stick is immersed in the FELUDA mix
  • Gold nanoparticle on the strip bind to the FELUDA complex
  • A protein called Streptavidin on the test line captures this gold nanoparticle bound-FELUDA complex
  • Unbound gold particles are captured on the control line
  • Colour develops on the test line and/or control line. One line indicates negative and two lines mean positive.
  • The test takes about one to two minutes