Mahendra Singh Dhoni won the toss on Monday and sent Australia in to bat in the fourth limited-overs international at Punjab Cricket Association Stadium.
India leads 2-1 in the seven-match series after winning back-to-back matches in Nagpur and Delhi.
Each team made a change to its line-up.
Australia included opener Shaun Marsh at the expense of Adam Voges, while Virat Kohli replaced injured batsman Gautam Gambhir in the Indian squad.
Virender Sehwag recovered from an injury sustained in the last game, passing fitness tests before being included in the team.
The Australian squad has been depleted by the hamstring injury that forced all-rounder James Hopes to return home on Sunday for recuperation. He became the third injured player after pace bowler Brett Lee and wicketkeeper Tim Paine to return to Australia from this tour.
