Scientists have finally unravelled the mystery behind a glassfrog's ability to turn transparent while they rest, according to a new study.
According to the study, a team of biologists and biomedical engineers has shown that glass frogs achieve transparency while resting by taking red blood cells from circulation and concealing them in their livers. The findings have been published in the journal Science.
It is easy to miss a glass frog in its natural environment. The northern glass frog, Hyalinobatrachium Fleischmanni, measures no more than a few centimeters, and they are most active at night when their green skin helps them blend in with the surrounding leaves and foliage.
But these amphibians become true masters of camouflage during the day when they're asleep.
"When glass frogs are resting, their muscles and skin become transparent, and their bones, eyes, and internal organs are all that's visible," said Carlos Taboada, co-first author of the paper.
"These frogs sleep on the bottoms of large leaves, and when they're transparent, they can perfectly match the colours of the vegetation," said Taboada.
Also Read | New study documents the songs of frog species
This photo provided by researchers in December 2022 shows a glass frog, a strict leaf-dwelling frog, that sleep, forage, fight, mate, and provide (male) parental care on leaves over tropical streams. Some frogs found in South and Central America have the rare ability to turn on and off their nearly transparent appearance, researchers report Thursday, December 22, 2022, in the journal Science. | Photo Credit: AP
Glass frogs are some of the only land-based vertebrates that can achieve transparency, which has made them a target for study. One reason transparency is so difficult to achieve is because of red blood cells in the circulatory system, the study said.
Red blood cells are adept at absorbing green light, which is the colour of light usually reflected by plants and other vegetation. In return, these oxygen-rich cells reflect red light, making blood - and by extension the circulatory system - highly visible, especially against a bright green leaf, the study said.
Taboada's team observed that red blood cells seemed to be disappearing from the circulating blood whenever the frogs became transparent. They conducted additional imaging tests on the animals, proving via optical models that the animals were able to achieve transparency because they were pushing red blood cells out of their vessels.
Taboada suspected that the cells were being stored in one of the frog's inner organs which are packaged in a reflective membrane, the study said.
"If these frogs are awake, stressed or under anaesthesia their circulatory system is full of red blood cells and they are opaque," explained Jesse Delia, co-author of the study.
Also Read | Do looks correlate with caregiving in frogs and toads?
"The only way to study transparency is if these animals are happily asleep, which is difficult to achieve in a research lab," said Delia.
But Taboada had learned about an imaging technology called photoacoustic microscopy, or PAM when he was studying biliverdin, the compound that gives certain species of frogs their signature green colour.
PAM involves shooting a safe laser beam of light into tissue, which is then absorbed by molecules and converted into ultrasonic waves. These sound waves are then used to make detailed biomedical images of the molecules.
The imaging tool is non-invasive, quiet, sensitive and, in a stroke of luck, available at Duke, the study said.
"PAM is the ideal tool for non-invasive imaging of red blood cells because you don't need to inject contrast agents, which would be very difficult for these frogs," explained Junjie Yao, an assistant professor of Biomedical Engineering at Duke who specializes in PAM technologies.
"The red blood cells themselves provide the contrast, because different types of cells absorb and reflect different wavelengths of light. We could optimize our imaging systems to specifically look for red blood cells and track how much oxygen was circulating in the frog's bodies," said Yao.
In their imaging set-up, the frogs slept upside down in a petri dish, similar to how they would sleep on a leaf, and the team shined a green laser at the animal. The red blood cells in the frog's body absorbed the green light and emitted ultrasonic waves, which were then picked up by an acoustic sensor to trace their whereabouts, with high spatial resolution and high sensitivity, the study said.
The results were startlingly clear: When the frogs were asleep, they removed nearly 90% of their circulating red blood cells and stored them in their liver, the study said.
"The primary result is that whenever glass frogs want to be transparent, which is typically when they're at rest and vulnerable to predation, they filter nearly all the red blood cells out of their blood and hide them in a mirror-coated liver - somehow avoiding creating a huge blood clot in the process," said Johnsen.
"Whenever the frogs need to become active again, they bring the cells back into the bloodstream, which gives them the metabolic capacity to move around," said Johnsen.
