Quicker, more reliable testing method

Sunayana Sarkar, graduate student at Manish Jaiswal’s lab, demonstrates the working principle of the LAMP assay.   | Photo Credit: By arrangement

Researchers at Tata Institute of Fundamental Research here have standardised a LAMP assay to rapidly detect the SARS-CoV 2 RNA among COVID patients in 30 minutes.

The RT-LAMP (reverse transcription loop-mediated isothermal amplification) assay has emerged as a viable alternative to meet the need for quicker and reliable testing methods since the RT-PCR based test which is the most accurate COVID-19 testing method was time consuming (six to eight hours for results) and required an expensive experimental set-up.

A release said the results of LAMP were determined by a change of colour of the sample once reagents were added. This was easily visible to the eye and aided in faster, easier detection. Contrary to RT-PCR based tests, this assay did not need experimental set-up.

The test was validated against both positive and negative COVID samples. The validation was carried out by K. Madhumohan Rao at NIMS and ESIC Hospital and Medical College.

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Printable version | Oct 23, 2020 4:02:20 PM | https://www.thehindu.com/news/cities/Hyderabad/quicker-more-reliable-testing-method/article32643973.ece

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